Botany, Gene Regulation, Molecular Biology, Signals and Responses
The Postdoctoral Research Associate will work on a three year Plant Genome Research Project funded by the NSF (IOS 1733633). The project is an inter-institutional research project between The University of Toledo’s Department of Biological Sciences, Michigan State University’s Department of Plant Biology, The University of Minnesota's College of Biological Sciences and the University of Wisconsin-Madison's Department of Agronomy. This project will develop resources for the investigation of transcriptional genomics in corn (Zea mays or maize). The Toledo campus is taking the lead in expanding the transcription factor (TF) ORFeome (TFome) collection) and developing anti-serum for the characterization of maize transcription factors. The Postdoctoral Research Associate will use newly developed antisera to characterize the in vivobinding properties of plant TFs of interest using techniques such as ChIP-Seq and ChIP-PCR (chromatin Immunoprecipitation with high throughput sequencing or PCR. Analysis of ChIP-Seq data will require familiarity with The Postdoctoral Research Associate will also assist in expanding the current TFome collection and then employ it to screen for novel TF-promoter interactions using the Yeast-1-hybrid technique (Y1H) and purse follow-up investigations. The Postdoctoral Research Associate should have strong verbal and written communication skills and is expected to take a leading role in developing manuscripts for publication of their work in peer reviewed journals.
All applications must be made through the University of Toledo Human Resources website via the following weblink:
For questions about the position please contact Dr. John Gray via email: email@example.com
Test suitability of recently developed antisera for Transcription Factor (TF) sensitivity, specificity, and affinity purify as necessary.
Employ newly developed antisera to explore TF protein-DNA interactions (PDIs) in vivo using techniques such as ChIP-Seq and ChIP-PCR (including computational analysis of results).
Oversee the amplification, cloning, and clone validation of maize transcription factor (TF) genes by undergraduate students using recombinant DNA technology.
Employ existing maize TFome (~2000 existing clones) and new clones (+ ~150) to discover new regulators of plant genes by Yeast-One-hybrid (Y1H) technique and follow up validation characterization (e.g. by reporter assays)
Train and supervise undergraduates to assist in the work of the Postdoctoral Research Associate
Take a leading role in preparing manuscripts for publication based on research conducted
Education/experience/licensing: Ph.D. degree in Molecular Biology or related field is required.
1 year experience in a molecular biology lab is required.
Six Months experience with protein overexpression in E. coli and protein purification is required.
Six Months experience with Western/Immunoblotting blots is required.
Six Months experience with Recombinant DNA technology and amplification of DNA using the Polymerase Chain reaction (PCR) is required.
Communication and other skills: Able to communicate effectively with other laboratory workers (including supervision and training of undergraduate students) and present his/her results at lab meetings.
Able to write up their research findings in the English language in preparation for scientific publication
Preferably has experience in the analysis of large DNA and RNA sequence datasets
Familiarity with DNA analysis software, database searching (BLAST program), and database entry is preferred
Preferably has experience in propagating and harvesting plant tissues for molecular biology studies.
Experience in supervising of other workers is preferred
Additional Salary Information: Plus benefits
Internal Number: 42288
About University of Toledo
Principal Investigators's Research Interest: I am a plant molecular biologist with a current interest in regulomics in maize (corn). We have created a Transcription Factor (TF) collection of most of the TFs in the maize genome and it has been used to identify novel regulatory components of the phenylpropanoid pathway - a key secondary metabolic pathway in all plants governing anthocyanin, flavonoid, and lignin biosynthesis. Our current work aims in further dissecting the regulation of phenylpropanoid genes using chromatin-immunoprecipation approaches in vivo. The work will have translational value in all crop species.
The University of Toledo: The University of Toledo is a student-centered, public metropolitan research university with 20,500 students and picturesque campuses recognized nationally for their beauty. The University of Toledo is one of 14 state universities in Ohio. It was established in 1872 and became a member of the state university system in 1967. The University of Toledo and the Medical University of Ohio merged July 2006 to form the third-largest public university operating budget in the state. The University is accredited by The Higher Learning Commission.... The Biological Sciences Department is housed within the College of Natural Sciences and Mathematics and is home to the Plant Science Research Center (PSRC). The PSRC, housed in Wolfe Hall, undertakes basic research in plant biology with an emphasis on plant molecular biology, nutrition, pathology, bioremediation, and ecology. The PSRC instructs students from undergraduate through post-doctoral levels; develops, in collaboration with other academic, government and industrial partners, technologies for transfer to the public and private sectors; and serves as a regional and global resource for research in the plant sciences.